| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Tampa Bay | 17 | 10 | 5 | 2 | 28:15 | 13 | 35 | |
| 2. | Charleston | 17 | 8 | 3 | 6 | 32:24 | 8 | 27 | |
| 3. | Detroit | 16 | 8 | 3 | 5 | 23:15 | 8 | 27 | |
| 4. | Louisville City | 17 | 8 | 3 | 6 | 28:26 | 2 | 27 | |
| 5. | Pittsburgh | 17 | 7 | 2 | 8 | 19:20 | -1 | 23 | |
| 6. | Rhode Island | 15 | 6 | 4 | 5 | 25:18 | 7 | 22 | |
| 7. | Indy Eleven | 15 | 6 | 3 | 6 | 18:17 | 1 | 21 | |
| 8. | Miami FC | 18 | 5 | 6 | 7 | 22:29 | -7 | 21 | |
| 9. | Hartford Athletic | 16 | 4 | 8 | 4 | 12:14 | -2 | 20 | |
| 10. | Loudoun | 16 | 2 | 9 | 5 | 19:29 | -10 | 15 | |
| 11. | Birmingham | 16 | 2 | 8 | 6 | 17:21 | -4 | 14 | |
| 12. | Brooklyn | 16 | 3 | 4 | 9 | 17:27 | -10 | 13 | |
| 13. | Sporting Jax | 18 | 1 | 5 | 12 | 23:46 | -23 | 8 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | Orange County SC | 17 | 8 | 7 | 2 | 27:19 | 8 | 31 | |
| 2. | San Antonio | 17 | 7 | 7 | 3 | 22:19 | 3 | 28 | |
| 3. | FC Tulsa | 16 | 7 | 5 | 4 | 19:16 | 3 | 26 | |
| 4. | Colorado Springs | 16 | 7 | 4 | 5 | 28:23 | 5 | 25 | |
| 5. | New Mexico | 14 | 7 | 3 | 4 | 19:15 | 4 | 24 | |
| 6. | Sacramento Republic | 15 | 6 | 5 | 4 | 19:15 | 4 | 23 | |
| 7. | Lexington | 16 | 6 | 4 | 6 | 24:20 | 4 | 22 | |
| 8. | El Paso | 16 | 6 | 4 | 6 | 27:25 | 2 | 22 | |
| 9. | Las Vegas Lights | 16 | 6 | 3 | 7 | 24:26 | -2 | 21 | |
| 10. | Phoenix Rising | 17 | 5 | 6 | 6 | 23:24 | -1 | 21 | |
| 11. | Oakland Roots | 17 | 5 | 6 | 6 | 25:27 | -2 | 21 | |
| 12. | Monterey Bay | 17 | 4 | 3 | 10 | 17:27 | -10 | 15 |